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Parvathi, M. S.
- Full-Length Cloning and Characterization of Abiotic Stress Responsive CIPK31-Like Gene from Finger Millet, a Drought-Tolerant Crop
Abstract Views :206 |
PDF Views:132
Authors
K. N. Nagarjuna
1,
M. S. Parvathi
1,
R. S. Sajeevan
1,
V. Pruthvi
1,
H. M. Mamrutha
2,
K. N. Nataraja
1
Affiliations
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
Source
Current Science, Vol 111, No 5 (2016), Pagination: 890-894Abstract
Regulatory proteins such as protein kinases are known to play a crucial role in plant stress acclimation pathways. In this study, we report the identification and characterization of an abiotic stress responsive protein kinase called CBL Interacting Protein Kinase (EcCIPK31-like) from finger millet, a drought-tolerant crop. PCR-based approach was followed to clone 1350 bp coding region that encodes a 449 amino acid protein with a calculated molecular mass of 50.69 kDa. The conserved domain analysis revealed the presence of CIPK domain with 90% sequence similarity with fox tail millet SiCIPK31-like gene. We also report its upregulation under salinity, desiccation, oxidative and temperature stresses at seedling level in finger millet. Drought stress at wholeplant level significantly induced the expression of EcCIPK31-like, indicating that the gene is linked to drought signalling pathways. The stress-responsive nature of EcCIPK31-like to diverse stresses indicates that the gene could regulate multiple cellular tolerance traits and its further functional validation can highlight the relevance in abiotic stress acclimation in plants.Keywords
Abiotic Stress Signalling, Finger Millet, Full-Length Cloning, Protein Kinase.Full Text
- Grafting as a promising technique for popularizing marigold cultivation in bacterial wilt sick soils in humid tropics of Kerala, India
Abstract Views :173 |
PDF Views:95
Authors
Affiliations
1 Department of Floriculture and Landscaping, College of Agriculture, Kerala Agricultural University, Thrissur 680 656, IN
2 Agricultural Research Station, Mannuthy, Kerala Agricultural University, Thrissur 680 656, IN
3 Department of Plant Physiology, College of Agriculture, Kerala Agricultural University, Thrissur 680 656, IN
1 Department of Floriculture and Landscaping, College of Agriculture, Kerala Agricultural University, Thrissur 680 656, IN
2 Agricultural Research Station, Mannuthy, Kerala Agricultural University, Thrissur 680 656, IN
3 Department of Plant Physiology, College of Agriculture, Kerala Agricultural University, Thrissur 680 656, IN
Source
Current Science, Vol 120, No 9 (2021), Pagination: 1517-1519Abstract
Studies on grafting nine susceptible genotypes including five F1 hybrids and four varieties of African marigold was conducted by using two bacterial wilt resistant marigold lines (M-1 and M-2) as ischolar_mainstocks. It was evident from the present study that the F1 hybrids recorded better survival of grafts compared to varieties of African marigold. The greatest survival of grafts was recorded in hybrid Bhagwati grafted on M-1 ischolar_mainstock (60%), which was on par with Bhagwati grafted on M-2 (54%), Maria 91 grafted on M-1 (54%) and Maria 91 grafted on M-2 (50%). Irrespective of the scion genotypes, M-1 as ischolar_mainstock recorded better graft survival (34.22%) compared to a survival of 25.56% in case of M-2. And, irrespective of the ischolar_mainstock genotypes, the highest survival was recorded in Bhagwati (57%), on par with Maria 91 (52%).Keywords
Bacterial wilt, cleft grafting, marigold, ischolar_mainstock.Full Text
References
- Kumbar, S., Rootstock evaluation and grafting studies in brinjal (Solanum melongena L.). M.Sc. (Hort.) thesis, Kerala Agricultural University, Thrissur, 2019, p. 109.
- Narayanankutty, C., Sreelatha, U. and Jaikumaran, U., Grafting to combat soil-borne diseases in vegetables. Indian Hortic., 2015, 60(6), 9–10.
- Umesh, C., Sreelatha, U., Kurian, P. S. and Narayanankutty, C., Evaluation of African marigold (Targets erecta L.) genotypes for yield and resistance to bacterial wilt pathogen, Ralstonia solanacearum. J. Trop. Agric., 2018, 56(1), 86–91.
- Jeevan, U., Sreelatha, U., Kurian, P. S., Anupama, T. V. and Parvathi, M. S., Assessment of resistance against bacterial wilt in marigold genotypes under humid tropics. J. Trop. Agric., 2019, 57(2), 152–159.
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- Spanò, R., Ferrara, M., Gallitelli, D. and Mascia, T., The role of grafting in the resistance of tomato to viruses. Plants, 2020, 9(8), 1042.
- Aslam, A. et al., Comparative analysis of primary metabolites and transcriptome changes between ungrafted and pumpkin-grafted watermelon during fruit development. Peer J., 2020, 8, e8259.
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